Finally, vitamin B12 levels were measured in vitamin Dstep three deficient and vitamin D3 normal group, and then these two groups were divided into subgroups of their own.
Primary endpoints are as follows: (1) determining the 25-(OH) D3 level in the general population and in patients with CKD; (2) comparing the clinical and laboratory data regarding inflammation with levels of 25-(OH) D3; (3) evaluation of whether low and normal 25-(OH) D3 levels and inflammation could explain this potential association;
dos.dos. Biochemical Analysis
25-Hydroxyvitamin D3 levels were measured by high performance liquid chromatographic analysis performed with using a Zivak HPLC system (Gebze, Turkey) using a commercial 25-OH vitamin D3 kit (Recipe, Munich, Germany). The reference values were 10–50 ?g/L for winter, 20–120 ?g/L for summer seasons. A deficiency in 25-(OH) D3 level was considered as below 10 ?g/L.
Solution vitamin B12 and you can plasma PTH account had been counted which have chemiluminescence means from the Immulite 2000 (Diagnostic Activities Corp., La, USA).
dos.step 3. Statistical Research
Analytical analyses were did by the SPSS 18.0 app (SPSS Inc., il, IL, USA). Distribution of information are determined by Kolmogorov-Smirnov take to. Continuous variables was in fact expressed as median (minimum-maximum) and you will categorical parameters because frequency and you will percent. Continuous details were compared to the Mann-Whitney
ensure that you categorical details was in fact opposed using Pearson’s Chi-rectangular test. Linear family relations between a couple continuing parameters try examined because of the Spearman relationship investigation.
step 3. Overall performance
A total of 1897 subjects were included in this retrospective study. Patients that measured 25-(OH) D3 levels under 10 ?g/l were 598 (31.5%), the number of those between 10 and 21 ?g/L was 751 (39.5%), and the number of those over 21 ?g/L was 550 (28.9%), respectively, in the study group (Figure 1).
(3–121)] compared to men [ (3–201)]. Therefore, male and female patients was indeed divided into communities with regards to the presence of kidney incapacity. The outcome are described from inside the Dining tables 3 and you can cuatro.
There was no significant correlation between age and vitamin D3 deficiency in our study population. There were lower serum albumin levels in patients with vitamin D3 deficiency, but this was siti incontri per lavoratori vietnamiti not statistically significant (Table 3). Median serum creatinine levels were less in patients with vitamin D3 deficiency without renal failure than in participants with normal vitamin D3 levels without renal failure (Table 4).
3 deficiency and vitamin D3 normal in male and female patients without renal failure (Tables 1–4). There was no difference in the levels of albumin, CRP, ESR, and WBC in women with renal insufficiency, but there was significant difference between levels of serum albumin and ESR in male patients.
In CRP variable that was categorized as <6 mg/L and ?6 mg/L, there was no significant difference between CRP categories and 25-(OH) D3 levels ( ). The results are summarized in Table 5.
In CRP variable that was categorized as <6 mg/L and ?30 mg/L, there was no significant difference between these CRP categories and 25-(OH) D3 levels (
Then, all participants were included in the study, both 25-(OH) D3 and CRP variables taken as a numerical, and correlation analysis was performed. There was no correlation between the two groups (
CRP and vitamin D3 levels in men and women were different; therefore, similar analyses were repeated in men and women groups. Median CRP in vitamin D3 deficient group and vitamin D3 normal group showed no significant difference in the male and female patients.
When 25-(OH) D3 and CRP variables are taken as categorical variables and analyzing the relationships between variables, no significant correlation was found ( ).
| Median (Min–Max) | Median (Min–max) |